Genome-Scale Variant Interpretation

Automated Radiation Dose Estimation

Mission Statement

MutationForecaster® (mutationforecaster.com) is Cytognomix’s patented web-portal for analysis of all types of mutations – coding and non-coding- including interpretation, comparison and management of genetic variant data. It’s a fully automated genome interpretation solution for research, translational and clinical labs.

Run our world-leading genome interpretation software on your exome, gene panel, or complete genome (Shannon transcription factor and splicing pipelines, ASSEDA, Veridical) with the Cytognomix User Variation Database and  Variant Effect Predictor.  With our integrated suite of software products, analyze coding, non-coding, and copy number variants, and compare new results with existing or your own database.  Select predicted mutations  by phenotype using articles with CytoVisualization Analytics.  With Workflows,  automatically perform end-to-end analysis with all of our software products.  Download an 1 page overview of MutationForecaster® (link)

Subscribe and analyze your own data via the cloud or… Don’t want to run your own analyses on MutationForecaster®? Let us do it for you with our Bespoke Analysis Service.

Experience our suite of genome interpretation products through a free trial of MutationForecaster®. Once you register, we provide datasets from our peer-reviewed publications to evaluate these software tools.

Automated radiation biodosimetry

Ionizing radiation produces characteristic chromosome changes. The altered chromosomes  are known as dicentric chromosomes [DCs]). DC biodosimetry is approved by the IAEA for occupational radiation exposure, radiation emergencies, or monitoring long term exposures.  The DC assay can also monitor effects of interventional radiation therapies.

Cytognomix has developed  a novel approach to find DCs (TBME).  The Automated Dicentric Chromosome Identifier and Dose Estimator (ADCI) software  works on multiple platforms and uses images produced by any of the existing automated metaphase capture systems found in most cytogenetic laboratories. ADCI is now available for for trial or  purchase (link).  Or contact us for details (pricing).

ADCI* uses machine learning to distinguish monocentric and dicentric chromosomes (Try the Dicentric Chromosome Identifier web app). With novel image segmentation, ADCI has become a fully functional cytogenetic biodosimetry system. ADCI takes images from metaphase scanning systems,  selects high quality cells, identifies dicentric chromosomes, builds biodosimetry calibration curves, and estimates exposures.  ADCI fulfills the criteria established by the IAEA for accurate triage biodosimetry of a sample in less than an hour. The accuracy is comparable to an experienced cytogeneticist. Check out our online user manual: wiki.

We find and validate mutations and gene signatures that others cannot with advanced,  patented genomic bioinformatic technologies. Cytognomix continues our  long track record of creating technologies for genomic medicine. We anticipate and implement the needs of the molecular medicine and genomics communities.

Predict chemotherapy outcomes

Pharmacogenomic responses to chemotherapy drugs can be predicted by supervised machine learning of expression and copy number of relevant gene combinations. Since 2015,  CytoGnomix has used biochemical evidence to derive gene signatures from changes in gene expression in cell lines, which can subsequently be examined in patients that have been treated with the same drugs. We have derived signatures for 30 different commonly used drugs.  Try out out our online predictor:  https://chemotherapy.cytognomix.com. 

Quantifying responses to ionizing radiation with gene expression signatures.

Gene signatures derived by machine learning have low error rates in externally validated, independent radiation exposed data. They exhibit high specificity and granularity for dose estimation in humans and mice.  These signatures can be designed to avoid the effects of confounding, comorbidities which can reduce specificity for detecting radiation exposures. See: https://f1000research.com/articles/7-233/v2

Single copy genomic technologies

Latest Posts

March 13, 2018. Oral presentation on chemotherapy response in Best Poster session at ESHG 2018

On behalf of the Scientific Programme Committee of the European Conference of Human Genetics 2018 taking place in Milan, Italy from June 16 to June 19, 2018, we are pleased to inform you that the abstract entitled: ‘Comprehensive prediction of responses to chemotherapies by biochemically-inspired machine learning’ (Control No. 2018-A-2095-ESHG) was among the best scored […]

February 19, 2018. Article on genomic signature of radiation exposure

Manuscript describing accurate genomic signatures  of radiation exposure will be published shortly by F1000Research. Jonathan ZL Zhao, Eliseos J Mucaki, Peter K  Rogan. Predicting Exposure to Ionizing Radiation by Biochemically-Inspired Genomic Machine Learning, F1000Research, in press. Abstract: Background: Gene signatures derived from transcriptomic data using machine learning methods have shown promise for biodosimetry testing. These […]

February 7, 2018. Accepted presentations at EPR Biodose (Munich, June, 2018)

Ali, S, Li Y, Shirley B, Wilkins R, Flegal F, Rogan PK, Knoll JHM. Population scale biodosimetry with the Automated Dicentric Chromosome Identifier and Dose Estimator (ADCI) software system. [Platform] Rogan PK, Zhao JZL, and Mucaki EJ. Predicting exposure to ionizing radiation by biochemically-inspired genomic machine learning.[Poster] Li Y, Shirley B, Wilkins R, Flegal, F, […]

May 4, 2015. Comment on PMID 23348723. Prediction of mutant mRNA splice isoforms by information theory-based exon definition.

Peter Rogan 2015 May 04 6:14 p.m. The Logic and Formulation of Exon Definition for Splice and Splicing Regulatory Sites with Negative Information Content. PK Rogan, EJ Mucaki Update on: Mucaki EJ, 2013 and the Automated Splice Site and Exon Definition Analysis server (ASSEDA). In Mucaki EJ, 2013, we described a method of predicting the overall strength of an exon […]

Oct. 1, 2017. Comment on PubMed PMID 28949076: Rules and tools to predict the splicing effects of exonic and intronic mutations. In: PubMed Commons [Internet]. Bethesda (MD): National Library of Medicine; 2017 Sep 26

Peter Rogan2017 Oct 01 8:57 p.m. We would like to alert readers to the fact that information theory-based splicing mutation analysis has been used to analyze a wide range of variants (in/dels and SNVs) that affect splicing in introns and exons in peer reviewed studies. These tools have been used analyze mutations that alter branchpoint […]

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