Please contact us if you would like a copy for non-commercial use.
Our paper, “Radiation Dose Estimation by Automated Cytogenetic Biodosimetry” by Peter K. Rogan, Yanxin Li, Ruth Wilkins, Farrah N. Flegal, and Joan H. M. Knoll, has been accepted for publication in the journal Radiation Protection Dosimetry.
Figure 1. Representative processed metaphase image in ADCI:
The University of Western Ontario has issued a press release about our studies describing a unified framework for prioritization of mutations in breast and ovarian cancer.
Other press outlets:
Discovery Grant from Natural Sciences and Engineering Research Council received for “Unravelling the metaphase epigenome: Differences in DNA accessibility between homologous chromosomes.” Joan Knoll, Principal Applicant and Peter Rogan, Co-applicant have been awarded 5 years of funding (2016-21) for this project.
Our paper, which describes a generalized information theory-based approach for mutation analysis of protein-nucleic binding sites, has been published:
Mucaki, E, Caminsky N, Perri A, Lu R, Laederach A, Halvorsen, M, Knoll, JHM, Rogan PK. A unified analytic framework for prioritization of non-coding variants of uncertain significance in heritable breast and ovarian cancer, BMC Medical Genomics, 9:19, 2016. DOI: 10.1186/s12920-016-0178-5. (link to paper) (PubMed citation)
Through a pan-Canadian collaboration led by Greg Zaric, we have published:
Cost-effectiveness of using a gene expression profiling test to aid in identifying the primary tumour in patients with cancer of unknown primary. M B Hannouf, E Winquist, S M Mahmud, M Brackstone, S Sarma, G Rodrigues, P Rogan, J S Hoch and G S Zaric.
The Pharmacogenomics Journal advance online publication 29 March 2016; doi: 10.1038/tpj.2015.94 (Link)
Peer-reviewed article in Early View in the journal Human Mutation: Link
“A unified analytic framework for prioritization of non-coding variants of uncertain significance in heritable breast and ovarian cancer,” by
Eliseos J. Mucaki; Natasha G. Caminsky; Ami M. Perri; Ruipeng Lu; Alain Laederach; Matthew Halvorsen; Joan H.M. Knoll; and Peter K. Rogan
has been accepted for publication in the journal, BMC Medical Genomics.
A preprint of this article is currently available at BioRxiv: http://biorxiv.org/content/early/2015/11/11/031419.
Our new paper on interpretation of gene variants in inherited breast and ovarian cancer has been accepted for publication in the journal, Human Mutation as a Research Article.
“Prioritizing variants in complete Hereditary Breast and Ovarian Cancer (HBOC) genes in patients lacking known BRCA mutations,” by Natasha G. Caminsky1, Eliseos J. Mucaki1, Ami M. Perri1, Ruipeng Lu2, Joan HM. Knoll3,4 and Peter K. Rogan1,2,4,5.
1Department of Biochemistry, Schulich School of Medicine and Dentistry, Western University, London, Canada, N6A 2C1, 2Department of Computer Science, Faculty of Science, Western University, London, Canada, N6A 2C1, 3Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, Canada, N6A 2C1, 4Cytognomix Inc. London, Canada, 5Department of Oncology, Schulich School of Medicine and Dentistry, Western University, London, Canada, N6A 2C1
A preprint of this article is published at http://biorxiv.org/content/early/2016/02/09/039206
We have added new capabilities to Variant Effect Predictor. Exome sequencing reveals many variants that have little or no effect on phenotype. You can remove these variants in MutationForecaster with our new stringency filters. Different default levels of filtering are offered. These can also be customized based on allele frequencies, predicted SIFT, Polyphen, variant type (eg. synonymous change), or protein coding domain containing the variant.
Yanxin Li1, Joan H. Knoll2,3, Ruth Wilkins4, Farrah N. Flegal5, and Peter K. Rogan1,3* Automated Discrimination of Dicentric and Monocentric Chromosomes by Machine Learning-based Image Processing. Departments of 1Biochemistry, and 2Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Cytognomix Inc., 4Health Canada, and 5Canadian Nuclear Laboratories.
in the journal Microscopy Research and Technique.
Abstract: Dose from radiation exposure can be estimated from dicentric chromosome (DC) frequencies in metaphase cells of peripheral blood lymphocytes. We automated DC detection by extracting features in Giemsa-stained metaphase chromosome images and classifying objects by machine learning (ML). DC detection involves i) intensity thresholded segmentation of metaphase objects, ii) chromosome separation by watershed transformation and elimination of inseparable chromosome clusters, fragments and staining debris using a morphological decision tree filter, iii) determination of chromosome width and centreline, iv) derivation of centromere candidates and v) distinction of DCs from monocentric chromosomes (MC) by ML. Centromere candidates are inferred from 14 image features input to a Support Vector Machine (SVM). 16 features derived from these candidates are then supplied to a Boosting classifier and a second SVM which determines whether a chromosome is either a DC or MC. The SVM was trained with 292 DCs and 3135 MCs, and then tested with cells exposed to either low (1 Gy) or high (2-4 Gy) radiation dose. Results were then compared with those of 3 experts. True positive rates (TPR) and positive predictive values (PPV) were determined for the tuning parameter, sigma. At larger sigma, PPV decreases and TPR increases. At high dose, for sigma= 1.3, TPR = 0.52 and PPV = 0.83, while at sigma= 1.6, the TPR = 0.65 and PPV = 0.72. At low dose and sigma = 1.3, TPR = 0.67 and PPV = 0.26. The algorithm differentiates DCs from MCs, overlapped chromosomes and other objects with acceptable accuracy over a wide range of radiation exposures.
A preprint of the paper is available at bioRxiv: http://biorxiv.org/content/early/2016/01/19/037309
We have made some major improvements to the Cytognomix User Variation Database recently. These are described in this recent video by Shannon Brown, a software developer at our company: CUVD Video
MutationForecaster now generates comprehensive genome interpretation on-the-fly. The results from all of our gene variant interpretation modules (Shannon Splicing Mutation Pipeline, ASSEDA, VEP, and Veridical) can now be automatically processed by CytoVA to find mutated genes in the genome related to a particular phenotypes based on published literature. Results are also be immediately processed to find dysfunctional biochemical pathways common to multiple mutated genes. All of the results are directly imported to your own CUVD repository, where all the results for each variant are grouped together.
The process is completely unattended. Start the Workflow for an variant set from an exome or genome sequence; several hours later all of the analyses are finished for you to review in your own CUVD database.