{"id":44,"date":"2011-03-17T16:03:21","date_gmt":"2011-03-17T16:03:21","guid":{"rendered":"http:\/\/localhost:14696\/?page_id=44"},"modified":"2025-11-27T20:20:58","modified_gmt":"2025-11-27T20:20:58","slug":"technology","status":"publish","type":"page","link":"https:\/\/www.cytognomix.com\/?page_id=44","title":{"rendered":"Single Copy Technology"},"content":{"rendered":"<table cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td width=\"712\" height=\"452\">\n<div>\n<p>Chromosomal  rearrangements are frequently monitored by fluorescence in situ  hybridization (FISH) using large, recombinant DNA probes consisting of  contiguous genomic intervals that are often distant from disease loci.  The Company has developed smaller, targeted, single-copy probes directly  from the human genome sequence. The primary applications are in  congenital disease and cancer diagnosis, but there are other emerging  applications for which these probes are well suited (stem cell  characterization, for example). These probes detect much smaller  abnormalities than conventional FISH probes, delineate chromosome  abnormality breakpoints, and can be targeted to specific regions within  genes.<\/p>\n<p>Probe sets have been designed genome-wide using our patented <em>abinitio<\/em>_probes<sup>TM<\/sup>. In addition to FISH, the company has designed oligonucleotide microarrays for array comparative genomic hybridization and solution capture arrays for targeted sequence enrichment prior to next generation sequencing. The aCGH arrays give more reproducible results than other commercial products. The capture arrays are designed for very high density coverage of inter- and intra-genic  sequences, including non-repetitive sequences that other sequence enrichment products avoid including. This produces better coverage for discovery of novel variants.<br \/>\n&nbsp;<br \/>\nThe  company produces both standard and custom genomic probes from  computationally defined, single-copy genomic sequences. Single-copy FISH  (SC-FISH) probes are produced directly from genomic DNA, and thus more  quickly than by recombinant DNA techniques. Probe sequences are inferred  from DNA sequences of larger genomic intervals&nbsp; with software that  determines the locations of repetitive DNA elements contained in these  sequences. The probes are 2-kb to 10-kb in length , synthesized in vitro, purified, and detected by FISH to chromosomes. The SC-FISH probes are then hybridized individually or in combination to human chromosomes.<br \/>\n&nbsp;<br \/>\nHybridization  results are analogous to conventional FISH, except that these shorter  probes can also be readily visualized. By contrast with conventional  FISH, pre-annealing or blocking with unlabeled, repetitive DNA is  unnecessary, as scFISH probes lack these sequences. Combinations of  probes from the same region can be designed to give single or multiple  hybridization signals on metaphase chromosomes.<br \/>\n&nbsp;<br \/>\nThe Company has developed SC FISH probes from many human chromosomal regions. More  than 200,000 such probes have been predesigned, covering most  anticipated diagnostic applications.&nbsp; In addition, over 500 SC FISH  probes have already been qualified on metaphase spreads.&nbsp; This  streamlined approach to the development and production of single-copy,  sequence-specific hybridization products is the key advantage of SC  Technology.<br \/>\n&nbsp;<\/p>\n<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Chromosomal rearrangements are frequently monitored by fluorescence in situ hybridization (FISH) using large, recombinant DNA probes consisting of contiguous genomic intervals that are often distant from disease loci. The Company has developed smaller, targeted, single-copy probes directly from the human genome sequence. The primary applications are in congenital disease and cancer diagnosis, but there are [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":[],"tags":[],"_links":{"self":[{"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=\/wp\/v2\/pages\/44"}],"collection":[{"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44"}],"version-history":[{"count":11,"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=\/wp\/v2\/pages\/44\/revisions"}],"predecessor-version":[{"id":5150,"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=\/wp\/v2\/pages\/44\/revisions\/5150"}],"wp:attachment":[{"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cytognomix.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}